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Bibliographic Data
Control Number7434
Date and Time of Latest Transaction20050414143248.3
General Information050127s2001 |||||||||b ||00|||
Cataloging SourcePCASTRD-DOST
Main Entry - Personal NameObena, Rofe-Amor P.
Title StatementBiologically Active Sterols and Triterpenses from the Leaves of Coleus Blumei Benth by Rofe-Amor P. Obena
Physical Descriptionxv, 118p. illus., graphs, plates, tables
Series Statement/Added Entry - TitleMaster of Science in Chemistry
Summary, Etc.Mixtures of sterols (FB1b3) and of triterpenes (FCI FIa) were isolated from the leaves of Coleus blumei Benth, locally known as mayana, a known folklore medicine for pain. The isolates exhibited analgesic, anti-inflammatory and antimicrobial activities.Using a bioassay-guided fractionation, this was the first study conducted to evaluate the analgesic activity of mayana extracts and the antifungal activity of the sterols. Also, this was the first report on the detection of campesterol, a-amyrin and ~-amyrin from mayana. GC-MS analysis showed that FB1b3 was a mixture of 6 components, of which three of the major components are sterols, namely: stigmasterol, ~-sitosterol, and campesterol. The sterol mixture (0.01 % yield from 1 Kg dried leaves) obtained from the active column fraction FB inhibited the acetic acid-induced pain by 39.0% at a dosage of 0.05 mg/g mouse. Analgesic experiments by oral ingestion and intraperitoneal injection were performed to compare the modes of administration and to determine the effect of dosage on analgesic activity. Intraperitoneal injection at a dosage of 0.05 mg/g mouse was found to be a more effective mode of administration. FB1b3 inhibited the carageenan-induced inflammation by 63.3% at a dosage of 0.1 mg/g mouse. At theconcentration of l~g/~L, FB1b3 inhibited weakly the growth of both C. albicans and T. mentagrophytes as shown by the antimicrobial index of 0.2 but was inactive towards E. coli and S. aureus. Similarly, FC1F1a was identified to be a mixture of the pentacyclic triterpenes a-amyrin and ~-amyrin with molecular mass of 426 g/mol and relative peak areas of 66.7% and 33.3%, respectively. The triterpene mixture (2.46 x 10-3% yield from 1 Kg driedleaves), also obtained from the active collmn FC fraction, inhibited the acetic acid- induced pain by 43.3% at a dosage of 0.05 mg/g mouse. It also inhibited the carageenan-induced inflammation by 30.8% at a dosage of 0.1 mg/g mouse but did not inhibit the microbial growth of E. coli, S. aureus, C. albicans and T. mentagrophytes at the concentration of 1 f-lg/f-lL.
Subject Added Entry - Topical TermChemistry -- Mayana
LocationDOST PCASTRD THESES Thesis 7000022 0 0000-00-00 N
  
     
 
Physical Location
Department of Science and Technology
Philippine Council for Advanced and Science Technology Research and Development
  
     
 
Digital Copy
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