Record Details     « New Search       Brief Record Full Record MARC Record     Bibliographic Data Control Number 334372 Date and Time of Latest Transaction 20180522074312.AM General Information 180522s |||||||||b ||00||| Cataloging Source STII-DOST Language Code eng Local Call Number Fil Q149 P5 N25 Main Entry - Personal Name Romero, Gabriel O.   Yaneshita, Makoto   Doan, Minhtam   Thomas, Bruce R.   Rodriguez, Raymond L. Title Statement Cloning and characterization of five β-glucanase genes in rice by Gabriel O. Romero, Makoto Yaneshita, Minhtam Doan, Bruce R. Thomas and Raymond L. Rodriguez Physical Description 236-252 figures, tables Summary, Etc. β-glucanases play key roles in the defense response of plants and in important physiological processes such as seed germination. As a step toward engineering better resistance and germination, we have cloned five β-glucanase genes in rice and characterized their structure and expression. Gns5 and Gns6 are tandemly arranged within a 6-kb region in the rice genome while Gns7, Gns8, and Gns9 are at least 8 kb apart from each other and from GnsS and Gns6. GnsS encodes a mature peptide of 304 aa, with an estimated pKI of 4.2. Gns6 species a mature peptide of 311aa, with an estimated pKI of 4.6. Gns7 encodes a mature peptide of 311 aa, with an estimated pKI of 5.4. Gns 8 specifies a mature peptide of at least 313 aa, with an• estimated pKI of 4.6. Gns9 encodes a mature peptide of 322 aa, with an estimated pKI of 9.9. The GnsS and Gns6 isozymes likely have a β-1,3-glucanase activity, while Gns7, Gns8, and Gns9 isozymes may have either a β-1,3-glucanase and/or a β-l, 3; l,4-glucanase activity or a novel substrate-specificity. Gns5 showed maximal expression in root and mature leaf, Gns6 in root and germinated seed, Gns7 in germinated seed, Gns8 in root, and Gns9 in calli and root. In young leaf. exogenous salicylic acid strongly induced GnsS, Gns6, and Gns7, and exogenous GA, auxin, ethylene, and ABA strongly induced Gns7 and Gns9 Subject Added Entry - Topical Term Science and technology   ABA   Auxin   β-Glucanase   Coleoptile   Ethylene   Seed germination   Rice -- Cloning and characterization   Agriculture           Physical Location           Digital Copy Not Available          Add to Book Cart  |   Download MARC