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Bibliographic Data
001
  
58097
005
  
20060619142816.3
008
  
060619s |||||||||b ||00|||
040
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$aPCARRD-DOST
100
1#
$aZamora, Alfinetta B.
130
00
$lENGLISH
245
00
$aTissue culture of banana.
260
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$bCollege, Laguna: University of the Philippines Los Banos, 1993. pp. 169-223. -(Sub proj. V).
500
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$aCollege, Laguna: University of the Philippines Los Banos, 1993. pp. 169-223. -(Sub proj. V).
520
3#
$aThe project aimed to improved the meristem culture technique in banana and its application in combination with heat treatment, virus elimination and germplasm conservation. Of the eight culture media found favorable for meristem culture, the medium developed by Vuylsteke was adopted. Culture vessel and volume of the culture medium did not affect the growth and development of meristems but were hastened with the use of preconditioned culture medium. In virus elimination studies, virus-free plantlets were obtained from infected meristem culture alone or in combination with heat treatment, depending on the virus infection. Heat treatment (40 deg C for 16 hours under light and 36 deg C for eight hours under darkness for four to eight weeks) of infected shoot culture prior to meristem excission was critical for elimination of banana bunchy top and banana bract mosaic virus infections. Meristem culture, by itself, produced virus-free plantlets from cucumber shoots infected with cucumber mosaic virus. Out of162 accessions in the vitro collection, 99 originated from the Philippines, 24 from Malaysia, 26 from Thailand, 12 from Indonesia and one from Nigeria.These were maintained with a modified propagation protocol. From the 317 samples of the 146 accessions which were indexed, 63 were free of BBTV, CMV and BBMV. These represented 63 virus-free accessions. Again, of the total collection, 95 cultivars were introduced to the minimal growth protocol. The maximum storage duration for the Philippine collection ranged from 8 to 36 months while those of the foreign accessions ranged from 6 to 23 months. Shoot culture maintained on the minimum growth medium were easily recovered, that is, resumption of growth or proliferation upon transfer to appropriate medium. Aside from using the minimal growth protocol with sugar alcohols, the medium with maleic hydrazide was also used to inhibit growth. Shoot proliferation was effectively reduced with cotton plugs, aluminum foil, rubber stoppers, polypropylene and plastic as vessel closures. Cryopreservation using protocols suitable for other crops and Musa embryos showed these protocols unsuitable for shoot tips and meristems of Musa. Shoot growth developed in explants were treated before immersion in liquid nitrogen but not after the liquid nitrogen treatment. All pretreatments did not adequately protect Musa shoot tips after treatment with liquid nitrogen using the paraffin technique.
650
04
$aBANANAS
650
04
$aTISSUE CULTURE
700
1#
$aAvenido, Renato A.
700
1#
$aDela Cruz, Felipe Jr. P.
700
1#
$aDamasco, Olivia P.
852
##
$aDOST$bPCARRD
991
##
$wREPORTS
  
     
 
Physical Location
Department of Science and Technology
Philippine Council for Agriculture, Forestry and Natural Resources Research and Development
  
     
 
Digital Copy
Not Available
  
     
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