Record Details     « New Search       Brief Record Full Record MARC Record     Bibliographic Data Control Number 409661 Date and Time of Latest Transaction 20201212061612.AM General Information 201212s |||||||||b ||00||| Cataloging Source DOST-PCHRD Main Entry - Personal Name Guillergan, Fred P   Coloso, Relicardo M   Abello, Myrna L   Valerta, Rosevale   Tayao, Lotgarda   Laganao, Chris Robinson   King, Jeremy John   Dolendo, Vanessa   Capuyan, Julierose   Cadiz, Maria Salud Laureen   Bistis, Christine Rio   Arceno, Ruby Liza   Agriam, Pamela Inocencia Title Statement Isolation and characterization of cytotoxic protein from the summer jellyfish (Cassiopea medusae) Physical Description 39-47 Summary, Etc. BACKGROUND: The summer jellyfish, Cassiopea medusae, is commonly seen in Philippine waters. Contact with these animals results to a wide spectrum of syndromes which range from localized irritation to death. The quantity of active compounds that can be isolated from the jellyfish makes it a target for search of anticancer agents OBJECTIVE: This study sought to isolate and characterize the cytotoxic proteins on nematocysts of jellyfish and to determine their potential as an anticancer drug. METHODS: This was a descriptive study using established biochemistry procedures. Isolation of the cytotoxic protein was determined using the brine shrimp bioessay. Aqueous extraction selectively precipitated the exchange chromatography and gel filtration chromatography. Protein concentration was determined by ultraviolet light spectrophotometry. RESULTS: The cytotoxic proteins of C. medusae were isolated and characterized to have molecular weights of 20 and 10 kDa, with an optimum pH of 7.0, optimum temperature of 4?C and an LC50 of 1microgram/ml. The cytotoxicity of the proteins has a variability inverse relationship to its molecular size, molecular weight, and protein concentration. CONCLUSION: The summer jellyfish contains cytotoxic proteins that can be isolated and purified for further studies on its possible use as an anticancer drug Subject Added Entry - Topical Term JELLYFISH   NEMATOCYST   CASSIOPEA MEDUSAE   CYTOTOXICITY   TOXIN   JELLYFISH   NEMATOCYST   CASSIOPEA MEDUSAE   CYTOTOXICITY   TOXIN           Physical Location           Digital Copy Not Available          Add to Book Cart  |   Download MARC