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MARC Record
Bibliographic Data
Control Number
39609
Date and Time of Latest Transaction
20160711061624.AM
General Information
160711s |||||||||b ||00|||
Cataloging Source
PSHS-DOST
Local Call Number
2002-26
Main Entry - Personal Name
Lucas, Rhea Therese M.
Lacsamana, May Cezanne D.
Chua, Peter Glenn Y.
Title Statement
Identification and Isolation of a competitive species versus
Aspergillus flavus
for the control aflatoxin production
Summary, Etc.
Aflatoxin, produced by Aspergillus flavus, is the most potent naturally-occurring carcinogen known. One alternative for preventing aflatoxin production is the inoculation of a competitive species against the producer of the mycotoxin, in this case, A. flavus. Test species (Trichoderma harzianum, Saccharomyces cerevisae, Penicillum notatum) and Aspergillus flavus were simultaneously inoculated into separate plates. After a certain incubation period, A. flavus was introduced to test species plates, and on the other hand, test species were introduced to correspding A. flavus plates. Microbiological assay was performed in order to determine the effect of the A. flavus and the test species on each other, in terms of growth, form, or aflatoxin production. The cultures were sent to the Food and Nutrition Research Institute for minicolumn testing which measures the levels of aflatoxin in each sample. It was observed that the test species Trichoderma harzianum was able to control the growth of Aspergillus flavus most effectively as compared to the other test species, such that no further growth of A. flavus was observed after it was inoculated into the plate culture of T. harzianum. As for the aflatoxin levels for each plate culture, the results showed negative presence of aflatoxin for every plate. The inhibiting effects of these test species in Aspergillus flavus growth is valuable as an alternative for other preventive measures against the production of aflatoxin and their spread into crops that could be ingested by humans and animals alike, and may result into damage of internal organs as well as cancer
Subject Added Entry - Topical Term
Aspergillus flavus -- Aflatoxin production
Trichoderma harzianum -- Aflatoxin production control
Location
DOST PSHS 2002-26 PROJECTS Research PSH-05-0055 1 4077 Complimentary 2002-04-01
Physical Location
Department of Science and Technology
Philippine Science High School
2002-26
Digital Copy
Not Available
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