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Bibliographic Data
Control Number
360986
Date and Time of Latest Transaction
20200116100252.AM
General Information
200116s |||||||||b ||00|||
Cataloging Source
STII-DOST
Local Call Number
(T) TD196.N48 D47 2017
Main Entry - Personal Name
De Padua, Jewel C.
Title Statement
Molecular identification and biosorption of nickel by marine and terrestrial strains of trichoderma by Jewel C. De Padua
Physical Description
xi, 88 leaves some colored figures, tables
Summary, Etc.
Nickel contamination is a serious environmental issue that requires immediate action. Microorganism such as fungi have been successfully used in the removal of toxic heavy metal ions in polluted environment, In this study,23 strains of Trichoderma were isolated from different terrestrial and marine substrata and identified using combined morphocultural and molecular methods. The identities of the fungi; strains were confirmed based on the gene sequence analysis of the ITS gene. The Trichoderma strains were also tested for their tolerance of heavy metals, and later for their biosorption of nickel. Our results (TSM) with 50 to 1,200 ppm of nickel (NiSO4). The highest radial growth was observed on TSM with nickel (NiSO4) at 50 ppm for three terrestrial and three marine strains for Trchoderma. More terrestrial strains(13) grew on this medium as compered to marine strains(8). Among the Fungal isolates, T. asperellum S03 isolated from soil exhibited the best growth after 2 days of incubation. For biosorption of nickel (NiSO4), the accumulation or uptake efficiency by selected 6 Trichoderma was determined in Potato Dextrose Broth supplemented with 50 ppm of nickel using Flame Atomic Absorption Spectrophotometer. The percent uptake efficiency of three strains of Trichoderma asperellum (S03,S08 and LL14) was computed to be 20-66% while Trichoderma virens (SG18 and SF22) achieved efficiency. However, Trichoderma inhamatum (MW25) also achieved 68% uptake the supernatant and Trichoderma asperellum LL14 had 15.82% retained nickel (NiSO4) in the supernatant. The 6 Trichoderma strains were also observed under the scanning electron microscopy to check surface morphology of the biomass before and after exposure to nickel (NiSO4). Scanning electron microscopy revealed no damage on the surface morphology of the Trichoderma biomass. Thus, our study showed the potential of terrestrial marine strains of Trichoderma for the bioremediation of heavy metals
Subject Added Entry - Topical Term
Environmental science
Nickel -- Molecular identification -- Biosorption
Molecular identification -- Nickel
Biosorption -- Nickel
Trichoderma
Nickel contamination
Location
DOST STII (T) TD196.N48 D47 2017 THESES T STI-18-0174 1 16-21084 Donation 1970-01-01
Physical Location
Department of Science and Technology
Science and Technology Information Institute
(T) TD196.N48 D47 2017
Digital Copy
Not Available
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