Record Details     « New Search       Brief Record Full Record MARC Record     Bibliographic Data Control Number 334408 Date and Time of Latest Transaction 20180522074312.AM General Information 180522s |||||||||b ||00||| Cataloging Source STII-DOST Language Code eng Local Call Number Fil Q149 P5 N25 Main Entry - Personal Name Matias, Ronald R.   Espiritu, Ma. Giselle V.   Buerano, Corazon C.   Natividad, Filipinas F.   Igarashi, Akira Main Entry - Corporate Name Dengue Research Network Title Statement Molecular detection and serotyping of dengue virus in Metro Manila, Philippines by Ronald R. Matias, Ma. Giselle V. Espiritu, corazon C. Buerano, Flipinas F. Natividad, Akira Igarashi and Dengue Research Network Physical Description 365-369 figure Summary, Etc. Dengue virus infection is a persistent health problem in the Philippines. Surveillance and diagnosis have been limited to recognizing the clinical symptoms. In this study, a reverse transcription-polymerase chain reaction (RT-PCR) assay complemented with virus isolation was used to detect and isolate dengue virus from sera of patients admitted to different medical institutions during the period 1995-1998. RT -PCR was done directly on serum samples. Additionally, serum samples were inoculated on Aedes albopictus cell.s and RT-PCR was done on infected culture fluid or on RNA extracted from infected cells. Dengue virus was detected and isolated in 95 out of 738 serum samples. Serotyping ofthe virus indicated the prevalence of Den 2 and Den 3 Subject Added Entry - Topical Term Serotyping of degue -- Metro Manila   Molecular detection   Dengue virus -- Metro Manila   Science and technology           Physical Location           Digital Copy Not Available          Add to Book Cart  |   Download MARC