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Bibliographic Data
Control Number
310827
Date and Time of Latest Transaction
20150706094049.AM
General Information
150706s |||||||||b ||00|||
Cataloging Source
STII-DOST
Local Call Number
ScienceDirect
Main Entry - Personal Name
Saif, T.A.
Kural, M.H.
Ahmed, W.W.
Title Statement
A novel platform for in situ investigation of cells and tissues under mechanical strain by W.W. Ahmed, M.H. Kural and T.A. Saif
Physical Description
pages 2979-2990 computer file; text; 2,921kb
Summary, Etc.
The mechanical micro-environment influences cellular responses such as migration, proliferation, differentiation and apoptosis. Cells are subjected to mechanical stretching in vivo, e.g., epithelial cells during embryogenesis. Current methodologies do not allow high-resolution in situ observation of cells and tissues under applied strain, which may reveal intracellular dynamics and the origin of cell mechanosensitivity. A novel polydimethylsiloxane substrate was developed, capable of applying tensile and compressive strain (up to 45%) to cells and tissues while allowing in situ observation with high-resolution optics. The strain field of the substrate was characterized experimentally using digital image correlation, and the deformation was modeled by the finite element method, using a Mooney-Rivlin hyperelastic constitutive relation. The substrate strain was found to be uniform for >95% of the substrate area. As a demonstration of the system, mechanical strain was applied to single fibroblasts transfected with GFP-actin and whole transgenic Drosophila embryos expressing GFP in all neurons during live imaging. Three observations of biological responses due to applied strain are reported: (1) dynamic rotation of intact actin stress fibers in fibroblasts; (2) lamellipodia activity and actin polymerization in fibroblasts; (3) active axonal contraction in Drosophila embryo motor neurons. The novel platform may serve as an important tool in studying the mechanoresponse of cells and tissues, including whole embryos
Subject Added Entry - Topical Term
Engineering
Fibroblasts -- Cytology
Microscopy, Atomic Force
Surface Properties
Location
DOST STII ScienceDirect NONPRINTS NP 14-15310 1 14-15310 Online/Download 2011-12-01
Physical Location
Department of Science and Technology
Science and Technology Information Institute
ScienceDirect
Digital Copy
Not Available
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