Record Details     « New Search       Brief Record Full Record MARC Record     Bibliographic Data Control Number 371173 Date and Time of Latest Transaction 20201212060927.AM General Information 201212s |||||||||b ||00||| Cataloging Source DOST-PCHRD Main Entry - Personal Name Buemio, Kristal Cielo K.   Lopez, Adelaine J.   Carandang, Rogie Royce Z. Title Statement The wound healing action of Kakawati gel from Gliricidia sepium (Jacques) Steudel (Family Fabaceae) Physical Description 0 Summary, Etc. Gliricidia sepium (Jacques) Steudel (Family Fabaceae), locally known as kakawati is recognized for its wound healing property. The study aimed to determine the wound healing action of a safe, stable, low cost formulation from the lyophilized sap of G sepium. The organoleptic, physical, and chemical properties were determined. Short term stability testing, as well as compatibility studies with common excipients were performed. The lyophilized sap extract was green and has a natural faint fetid odor. It was found to be stable at temperatures of 5, 30 and 50 degrees C. It was stable at pH values of 5 to10 and in natural and artificial light. After compatibility testing, it was found to be physically and chemically compatible with sodium CMC, glycerin, sodium citrate and sodium benzoate. The trial formulation that had the best organoleptic properties and a gel system that was found to be stable was chosen to be the prototype formula for the laboratory scale production. The formulated product is a green gel of moderate consistency possessing a natural fragrance with faint fetid odor. Quality control tests were performed on the raw materials as well as with the final product. A Master Batch Record for G sepium gel (7.5 percent) was prepared and gel specifications were established. The product was subjected to two light sources, different levels of moisture, and accelerated temperatures of 40, 50 and 60 degrees C. Physical properties and TLC profiles of the gel were noted. The gel was stable at 30 degrees C for the whole duration of testing. At high temperatures, the product showed degradation such as decreased in viscosity, darkening of color and disappearance of spots in the chromatograms. The gel was stable at 30 degrees C. The gel formulation was found to be effective at 7.5 percent concentration. Wound healing property was determined by three methods: (1) measurement of the rate of wound contraction; (2) tensile strength of the healed tissue using a tensiometer and (3) histological examination of collagen deposition. The 7.5 percent gel formulation was also tested for its efficacy with wound healing-impaired rats. As a wound healing promoter, it possesses coagulant activity. The determination showed reduction of bleeding time upon application of the gel on the cut tail. It is noteworthy that both the sap and formulated gel had activity against Tricophyton mentagrophytes, Candida albicans and Pseudomonas aeruginosa through antimicrobial screening. Skin irritation testing was also conducted and no signs of immediate irritation were observed. The gel formulation costs about PhP 20.98 per unit based on the product costing. This is relatively cheaper than the wound healing agents available in the market today. Therefore, the gel formulation has been proven to have wider application than being a wound healing promoter alone and found to be safe, effective, stable and low cost. (Author) Subject Added Entry - Topical Term WOUND HEALING   FABACEAE   KAKAWATI   GLIRICIDIA SEPIUM   HEALING PROPERTY   WOUND HEALING   FABACEAE           Physical Location           Digital Copy Not Available          Add to Book Cart  |   Download MARC