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Control Number310269
Date and Time of Latest Transaction20150706095140.AM
General Information150706s |||||||||b ||00|||
Cataloging SourceSTII-DOST
Local Call NumberScienceDirect
Main Entry - Personal NameDivecha, Jyoti
 Jog, Rahul
 Mohana, Sarayu
 Acharya, Bhavik K.
 Madamwar, Datta
Title StatementUtilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation by Bhavik K. Acharya and 4 others
Physical Descriptionpages 2019-2027 computer file; text; 403kb
Summary, Etc.Pollution caused by distillery spent wash on one hand has stimulated the need to develop new technologies to treat the waste and on the other, forced us to reevaluate the efficient utilization of its nutritive potential for production of various high value compounds. In this study, anaerobically treated distillery spent wash was used for the production of cellulases by Aspergillus ellipticus under solid-state fermentation using wheat straw as a substrate. The interactions between distillery effluent concentration, initial pH, moisture content and inoculum size were investigated and modeled using response surface methodology (RSM) involving Box-Behnken design (BBD). Under optimized conditions, filter paper activity, beta-glucosidase and endo-beta-1,4-glucanase activities were found to be 13.38, 26.68 and 130.92 U/g of substrate respectively. Characterization of endo-beta-1,4-glucanase and beta-glucosidase was done after partial purification by ammonium sulfate fractionation followed by desalting. The partially purified endo-beta-1,4-glucanase and beta-glucosidase showed maximum activity at 60 degrees C. Saccharification studies performed with different lignocellulosic substrates showed that wheat bran was most susceptible to enzymatic hydrolysis. The study suggests that anaerobically treated distillery spent wash can be used as a viable nutrient source for cellulase production under solid-state fermentation by A. ellipticus
Subject Added Entry - Topical TermBiology
 Ammonium Sulfate -- Chemistry
 Cellulases -- Biosynthesis
 Hydrogen-Ion Concentration
 Chemical Fractionation
LocationDOST STII ScienceDirect NONPRINTS NP 14-15028 1 14-15028 Online/Download 2010-12-01
 
     
 
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Department of Science and Technology
Science and Technology Information InstituteScienceDirect
 
     
 
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