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MARC Record
Bibliographic Data
Control Number
310269
Date and Time of Latest Transaction
20150706095140.AM
General Information
150706s |||||||||b ||00|||
Cataloging Source
STII-DOST
Local Call Number
ScienceDirect
Main Entry - Personal Name
Divecha, Jyoti
Jog, Rahul
Mohana, Sarayu
Acharya, Bhavik K.
Madamwar, Datta
Title Statement
Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation by Bhavik K. Acharya and 4 others
Publication, Distribution, Etc.
Amsterdam Elsevier Ltd. 2010
Physical Description
pages 2019-2027 computer file; text; 403kb
Bibliography, Etc. Note
Includes bibliographical references
Summary, Etc.
Pollution caused by distillery spent wash on one hand has stimulated the need to develop new technologies to treat the waste and on the other, forced us to reevaluate the efficient utilization of its nutritive potential for production of various high value compounds. In this study, anaerobically treated distillery spent wash was used for the production of cellulases by Aspergillus ellipticus under solid-state fermentation using wheat straw as a substrate. The interactions between distillery effluent concentration, initial pH, moisture content and inoculum size were investigated and modeled using response surface methodology (RSM) involving Box-Behnken design (BBD). Under optimized conditions, filter paper activity, beta-glucosidase and endo-beta-1,4-glucanase activities were found to be 13.38, 26.68 and 130.92 U/g of substrate respectively. Characterization of endo-beta-1,4-glucanase and beta-glucosidase was done after partial purification by ammonium sulfate fractionation followed by desalting. The partially purified endo-beta-1,4-glucanase and beta-glucosidase showed maximum activity at 60 degrees C. Saccharification studies performed with different lignocellulosic substrates showed that wheat bran was most susceptible to enzymatic hydrolysis. The study suggests that anaerobically treated distillery spent wash can be used as a viable nutrient source for cellulase production under solid-state fermentation by A. ellipticus
Subject Added Entry - Topical Term
Biology
Ammonium Sulfate -- Chemistry
Cellulases -- Biosynthesis
Hydrogen-Ion Concentration
Chemical Fractionation
Location
DOST STII ScienceDirect NONPRINTS NP 14-15028 1 14-15028 Online/Download 2010-12-01
Physical Location
Department of Science and Technology
Science and Technology Information Institute
ScienceDirect
Digital Copy
Not Available
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