Home
|
About
|
Membership
|
Register
|
Contact Us
|
Site Links
|
Site Map
|
Login
Record Details
«
New Search
Brief Record
Full Record
MARC Record
Bibliographic Data
Control Number
7429
Date and Time of Latest Transaction
20050414143247.6
General Information
050127s2000 |||||||||b ||00|||
Cataloging Source
PCASTRD-DOST
Main Entry - Personal Name
Mag-aso, Fema Y.
Title Statement
Detection of Dehyrogenase Genes from Ciliates using Polymerase Chain Reaction Technique by Fema Y. Mag-aso
Physical Description
117p. illus., graphs, plates, tables
Series Statement/Added Entry - Title
Master of Science in Biology
Summary, Etc.
Six primer pairs were designed from the biphenyl (bph) operon of Pseudomonas pseudoalcaligenes KF707 to detect dehydrogenase genes from ciliates using polymerase chain reaction (PCR) technique. Optimum PCR conditions were obtained empiricallyand had much lower annealing temperatures than those suggested by the OLIGO 4.0-s primer design program. Four (4) PCR products were obtained from 3 out of6 species of ciliates used in the study. Two (2) products, AIL and Al Tm of primer bphAI are fromParamecium aurelia complex (L) and from Tetrahymena sp. (Tm), respectively. BTI and CTI, both from Tetrahymena thermophila (TI) are products of primers bphB and bphC, respectively. Analysis of the sequenced PCR products using BLASTN and F ASTXY programs strongly suggests that AlL, Al Tm and BTI sequences are fragments of dehydrogenase genes. These are not only potential PCB-degrading genes but may also be involved in the degradation pathway of other xenobiotics. Among the 3 sequences, the BTI sequence had perfect annealing sites for both the sense and anti-sense primers. The CTI sequence differs from the other products as shown by BLASTN and F ASTXY search in that it is 100% homologous to dynein heavy chain of 7: thermophila.
Subject Added Entry - Topical Term
Biology -- Pseudomonas pseudoalcaligenes
Location
DOST PCASTRD THESES Thesis 7000017 0 0000-00-00 N
Physical Location
Department of Science and Technology
Philippine Council for Advanced and Science Technology Research and Development
Digital Copy
Not Available
Add to Book Cart
|
Download MARC
Online Catalog
Basic Search
Advanced Search
Browse Subjects
Book Cart
Text Size:
S
-
M
-
L
Home
|
About
|
Membership
|
Register
|
Contact Us
|
Site Links
|
Site Map
|
Login
Copyright © 2004-2025. Philippine eLib Project
Host: U.P. Diliman University Library
