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Control Number315387
Date and Time of Latest Transaction20160315064424.AM
General Information160315s |||||||||b ||00|||
Cataloging SourceSTII-DOST
Local Call Number(T) QK626 A97 2004
Main Entry - Personal NameAurin, Cathedrine Le Minh
Title StatementDevelopment of cloning and transformation systems in the pleuromutilin-producing basidiomycete clitopilus passeckerianus (Pilat) singer NRRL 3100 by Cathedrine Le Minh Aurin
Physical Descriptiona162 leaves colored figures, tables
Summary, Etc.aCloning and transformation systems were developed in the basidiomycete clitopilus passeckerianus NRRL 3100. Five strains of C. passeckerianus were used, namely the wild type strain (Cpwt), HP76, LP1, LP2 and LP5. Genomic DNA was isolated from the five strains and used as template for polymerase chain reaction (PCT. The primers used were constructed based on GENBANK sequences of the geranylgeranyltransferase (GGT) gene, one of the genes involved in the biosynthetic pathway of pleuromutilin. A 200-bp product was obtained from primer pairs GGT2F/GGT2R and GGT3F/GGT3R using Cpwt as template. Degenearate primers based on GGT were also constructed. From the degenerate primers, five bands were obtained from Cpwt, eight bands for LP5, while no PCR products were obtained from HP76, LP1 and LP2.0
Subject Added Entry - Topical TermaBiology0
 aCloning0
 aPolymerase chain reaction (PCR)
LocationaDOST STII (T) QK626 A97 2004 THESES T STI-13-2258 1 14-16891 Donation 2013-05-20
  
     
 
Physical Location
Department of Science and Technology
Science and Technology Information Institute(T) QK626 A97 2004
  
     
 
Digital Copy
Not Available
  
     
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