Record Details     « New Search       Brief Record Full Record MARC Record     Bibliographic Data Control Number 409974 Date and Time of Latest Transaction 20201212061612.AM General Information 201212s |||||||||b ||00||| Cataloging Source DOST-PCHRD Main Entry - Personal Name delos Reyes, Amor M   Merca, Florinia E Title Statement Isolation and characterization of a lectin from Bacillus thuringiensis subs. Morrisoni (Serotype H8a:8b) PG-14 Physical Description 1-17 Summary, Etc. A lectin which is mitogenic against human peripheral lymphocytes and has relatively weak mosquitocidal activity was isolated from the spores/crystals of B thuringiensis subsp. morrisoni (serotype H 8a:8b) PG-14. The lectin was isolated by solubilizing the spores/crystals in alkaline pH in the presence of dithiothreitol. Purification was accomplished by ammonium sulfate fractination and gel chromatography using sephadex G-200. The purified lectin was found to be homogeneous by polyacrylamide gel electrophoresis under non-denaturing condition at pH 8.8. The lectin was found to have no blood group and blood type specificity since it agglutinated all human blood types (A,B,)) and animal blood (calf and goat) used in the experiment. Hapten inhibition test did not show any sugar specificity based from the standard sugars used. The purified lectin is a glycoprotein with a total sugar content of 10.96% as determined by the phenol sulfuric acid method. The approximate molecular weight of the single polypeptide band obtain from sodium-dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was calculated to be 65 kD. Morphological examination of human lymphocyte cultured in the lection showed that 43% of the cell population had been transformed after 72 hours indicating the mitogenecitiy of the lectin. Insect toxicity assay using 3-day oldf aedes albopictus larvae resulted to a relatively lower mortality rate after 24-hr exposure to the lectin compared to the crude extract Subject Added Entry - Topical Term BACILLUS THURINGIENSIS   ISOLATION AND CHARACTERIZATION   LECTIN   BACILLUS THURINGIENSIS           Physical Location           Digital Copy Not Available          Add to Book Cart  |   Download MARC